Frédéric JAMME

Synchrotron SOLEIL

Frédéric JAMME

Synchrotron SOLEIL

Résumé

SHINING SYNCHROTRON LIGHT INTO CELLS AND TISSUES

 Frederic Jamme, Ferenc Borondics, Christophe Sandt, Matthieu Réfrégiers

Synchotron SOLEIL, L’Orme des Merisiers, 91190 Saint-Aubin, Gif-sur-Yvette, France

 

Since the early 90s and more specifically during the last decade, infrared microspectroscopy at synchrotron facilities has been widely exploited. The main reasons for the utilization infrared light are enhanced lateral resolution (typically at or very close to the far field diffraction limit), superior signal to noise ratio obtained with short acquisition times, and wide energy range availability. At the SOLEIL synchrotron, the SMIS beamline (Spectroscopy and Microscopy in the Infrared using Synchrotron) is operational since January 2008, and recently important upgrade including nano-IR and IR tomography is being developed.

In close collaboration with the SMIS beamline, the DISCO beamline (Dichroism, Imaging and Spectroscopy for Chemical and biOlogy) uses synchrotron deep ultraviolet (DUV) fluorescence to open up new possibilities in biology and in particular in drug delivery and nano-biotechnology. Indeed, DUV autofluorescence does not need external specific probes or labelling and profits from the intrinsic fluorescence that exist for many biomolecules when excited in this wavelength range. Thus, two DUV fluorescence microscopes set-up for biology have been developed to provide fine tuneable excitation from 200 to 600 nm. We have demonstrated, that using the right experimental conditions, auto fluorescence background signal is rich in information and can become useful in further understanding biological.

The latest technical developments and various applications to biological systems will be presented from both the SMIS and the DISCO beamlines at SOLEIL synchrotron.

 

  1. Dumas, P., Polack, F., Lagarde, B., Chubar, O., Giorgetta, J., and Lefrancois, S. (2006), Infrared Physics & Technology 49, 152–160.
  2. . Giuliani, F. Jamme, V.Rouam, F. Wien, J.L. Giorgetta, B. Lagarde,O. Chubar,S. Bac, I. Yao,S. Rey, C. Herbeaux, J.L Marlats, D. Zerbib, F. Polack and M. Réfrégiers, J. Synchrotron Rad. 2009, 16: 835- 841.
  3. Jamme, F., Villette, S., Giuliani, A., Rouam, V., Wien, F., Lagarde, B., & Refregiers, M. Microscopy and Microanalysis, 2010, 16(5): 507-514.
  4. Jamme, Kascakova, S., Villette, S., Allouche, F., Pallu, S., Rouam, V., & Réfrégiers, M. Biology of the Cell,, 2013,105(7), 277–288.
  5. Petit VW, Réfrégiers M, Guettier C, Jamme F, Sebanayakam K, Brunelle A, Laprévote O, Dumas P and Le Naour F. 2010. Multimodal spectroscopy combining time-of-flight-secondary ion mass spectrometry, synchrotron-FT-IR, and synchrotron-UV microspectroscopies on the same tissue section., Analytical Chemistry 82: 3963–3968.
  6. Allouche F, Hanafi M, Jamme F, Robert P, Barron C, Guillon F and Devaux MF. 2012. Coupling hyperspectral image data having different spatial resolutions using Multiple Co-inertia Analysis. Chemometrics and Intelligent Laboratory Systems. 117 : 200-212.

 

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