Vincent STUDER

IINS (Bordeaux)

Vincent STUDER

IINS (Bordeaux)

Résumé

Multi-Protein printing by Light-Induced Molecular Adsorption

Vincent Studer
University of Bordeaux, Interdisciplinary Institute for Neuroscience, Bordeaux, France.
CNRS UMR 5297, F-33000 Bordeaux, France

 

Molecular printing has developed tremendously in the past few years. Established protein patterning methods either rely on a slow serial writing process or are based on parallelized photolithographic techniques where cumbersome mask alignment procedures usually impair the ability to generate multi-protein patterns. Here we describe how to perform high resolution multi protein micro-patterning using Light Induced Molecular Adsorption of Proteins (LIMAP). LIMAP is based on a water-soluble photo-initiator which is able to reverse the antifouling property of polymer brushes when exposed to UV light. We show that the density of adsorbed molecules scales almost linearly with the dose of UV light. Moreover LIMAP is well adapted to wide field illumination schemes. Consequently, micron-scale adhesive patterns where proteins can adsorb, are printed within seconds. The very low background of these patterns allows for the sequential printing of multiple proteins. Our optical set-up (widefield DMD based projection system coupled to a conventional epifluorescence microscope) allows us to generate arbitrary grayscale patterns of UV light. Patterns ranging from 500nm to 1 mm can be printed in one step and for a given fluorescent protein controlled gradients of arbitrary shape can be patterned. We show that the range of application extends from the single molecule up to the multicellular scale with an exquisite control over local protein density: micropatterns of individual fluorescent molecule provide a tool for the quantitative study of biomolecular interactions. At larger scale, the ability to rapidly generate complex and dynamic protein landscapes enable studies of the cell-cell and cell-matrix interactions. Altogether, LIMAP provides a generic method for fast high resolution patterning of multiple protein with applications to biomedical research and tissue engineering.

 

Strale, PO., Azioune, A., Bugnicourt, G., Lecomte, Y., Chahid, M., Studer, V.
Multiprotein Printing by Light-Induced Molecular Adsorption. Adv. Mater. 2016, 28, 2024–2029

 

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